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The decrease in cell viability was almost completely rescued by either iron chelation via deferoxamine (DFO) or by the lipophilic antioxidant ferrostatin- 1 (Fer1), demonstrating that cell death is both iron and reactive- oxygen species (ROS) dependent

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While not fully understood, the analgesic effects are believed to be due to the activation of the descending serotonergic inhibitory pathways in the central nervous system