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Researchers should consider: Temperature Light exposure Container compatibility Number of freeze-thaw cycles Time in solution Sterility requirements appropriate to the experiment Compound-specific stability data Avoid unnecessary freeze-thaw cycling Repeatedly freezing and thawing a research solution can increase the risk of chemical and physical degradation

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The mild procedure (minimally invasive lumbar decompression) and epidural steroid injections are both common treatment options for lumbar spinal stenosis